Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
نویسندگان
چکیده
Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier transform infrared (FTIR) imaging of cellular chemistry in living cells a reality. The availability of bright, broadband IR sources and large area, pixelated detectors facilitate live cell imaging, which requires rapid measurements using non-destructive probes. In this work, we review advances in the field of FTIR spectromicroscopy that have contributed to live-cell two and three-dimensional IR imaging, and discuss several key examples that highlight the utility of this technique for studying the structure and chemistry of living cells.
منابع مشابه
An overview of clinical PET/CT
This article is intended to provide an overview of various aspects of clinical PET/CT. These include discussions of: (i) Important areas of clinical application; (ii) Opportunities in clinical research; (iii) Scanner and operating-mode considerations (e.g. BGO vs. LSO, LYSO or GSO scanners, 2D vs. 3D imaging). (iv) Study-specific considerations (e.g. patient preparation and positioning is...
متن کاملA Comparative Study of VHDL Implementation of FT-2D-cGA and FT-3D-cGA on Different Benchmarks (RESEARCH NOTE)
This paper presents the VHDL implementation of fault tolerant cellular genetic algorithm. The goal of paper is to harden the hardware implementation of the cGA against single error upset (SEU), when affecting the fitness registers in the target hardware. The proposed approach, consists of two phases; Error monitoring and error recovery. Using innovative connectivity between processing elements ...
متن کامل3D high-density localization microscopy using hybrid astigmatic/ biplane imaging and sparse image reconstruction.
Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs), imaging of densely activated molecules can improve the limited temporal resolution, as has been well demonstrated in two-dimensional imagi...
متن کاملTowards analytically useful two-dimensional Fourier transform ion cyclotron resonance mass spectrometry.
Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) achieves high resolution and mass accuracy, allowing the identification of the raw chemical formulae of ions in complex samples. Using ion isolation and fragmentation (MS/MS), we can obtain more structural information, but MS/MS is time- and sample-consuming because each ion must be isolated before fragmentation. In 1987,...
متن کاملEvaluation of a fast method of EPID-based dosimetry for IMRT and Comparison with 3D EPID-based dosimetry system using conventional two- and three-dimensional detectors for VMAT
Introduction: Electronic portal imaging devices (EPIDs) could potentially be useful for intensity-modulated radiation therapy (IMRT) and VMAT QA. The data density, high resolution, large active area, and efficiency of the MV EPID make it an attractive option. However, EPIDs were designed as imaging devices, not dosimeters, and as a result they do not inherently measure dose in ...
متن کامل